SBL540: Difference between revisions
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| credit_structure = 3-0-2 | | credit_structure = 3-0-2 | ||
| pre_requisites = | | pre_requisites = | ||
| overlaps = SBL721 | | overlaps = [[SBL721]] | ||
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== SBL540 : Experimental Methods in Biology == | == SBL540 : Experimental Methods in Biology == | ||
Courses of Study 2024-2025 Biological Sciences 350Introduction to biomolecules and cells; Electrophoresis, DNA fingerprinting and EMSA; Basics of microscopy; Optical Spectroscopy – Raman, IR, Fluorescence, UV-VIS; FCS; Electron Microscopy; NMR; Circular Dichroism; Force Spectroscopy – AFM, Optical Tweezers, Magnetic Tweezers; Surface Plasmon Resonance, Mass spectrometry, Chromatography, X-Ray Crystallography, Flow-Cytometry, Recent advances in biophysical techniques – HiTS-FLIP, Mass Photometry, DNA nanotechnology Laboratory: Separation of nucleic acid and proteins on agarose and polyacrylamide gel electrophoresis respectively; difference between native and SDS-PAGE; protein purification using affinity and size exclusion chromatography; molecular weight calculation from SEC; protein purity and secondary structure assessment using CD spectroscopy; characterizing cells or biomolecules using Raman spectroscopy. | Courses of Study 2024-2025 Biological Sciences 350Introduction to biomolecules and cells; Electrophoresis, DNA fingerprinting and EMSA; Basics of microscopy; Optical Spectroscopy – Raman, IR, Fluorescence, UV-VIS; FCS; Electron Microscopy; NMR; Circular Dichroism; Force Spectroscopy – AFM, Optical Tweezers, Magnetic Tweezers; Surface Plasmon Resonance, Mass spectrometry, Chromatography, X-Ray Crystallography, Flow-Cytometry, Recent advances in biophysical techniques – HiTS-FLIP, Mass Photometry, DNA nanotechnology Laboratory: Separation of nucleic acid and proteins on agarose and polyacrylamide gel electrophoresis respectively; difference between native and SDS-PAGE; protein purification using affinity and size exclusion chromatography; molecular weight calculation from SEC; protein purity and secondary structure assessment using CD spectroscopy; characterizing cells or biomolecules using Raman spectroscopy. | ||
Latest revision as of 16:45, 14 April 2026
| SBL540 | |
|---|---|
| Experimental Methods in Biology | |
| Credits | 4 |
| Structure | 3-0-2 |
| Pre-requisites | |
| Overlaps | SBL721 |
SBL540 : Experimental Methods in Biology
Courses of Study 2024-2025 Biological Sciences 350Introduction to biomolecules and cells; Electrophoresis, DNA fingerprinting and EMSA; Basics of microscopy; Optical Spectroscopy – Raman, IR, Fluorescence, UV-VIS; FCS; Electron Microscopy; NMR; Circular Dichroism; Force Spectroscopy – AFM, Optical Tweezers, Magnetic Tweezers; Surface Plasmon Resonance, Mass spectrometry, Chromatography, X-Ray Crystallography, Flow-Cytometry, Recent advances in biophysical techniques – HiTS-FLIP, Mass Photometry, DNA nanotechnology Laboratory: Separation of nucleic acid and proteins on agarose and polyacrylamide gel electrophoresis respectively; difference between native and SDS-PAGE; protein purification using affinity and size exclusion chromatography; molecular weight calculation from SEC; protein purity and secondary structure assessment using CD spectroscopy; characterizing cells or biomolecules using Raman spectroscopy.