SBL540
| SBL540 | |
|---|---|
| Experimental Methods in Biology | |
| Credits | 4 |
| Structure | 3-0-2 |
| Pre-requisites | |
| Overlaps | SBL721 |
SBL540 : Experimental Methods in Biology
[edit]Courses of Study 2024-2025 Biological Sciences 350Introduction to biomolecules and cells; Electrophoresis, DNA fingerprinting and EMSA; Basics of microscopy; Optical Spectroscopy – Raman, IR, Fluorescence, UV-VIS; FCS; Electron Microscopy; NMR; Circular Dichroism; Force Spectroscopy – AFM, Optical Tweezers, Magnetic Tweezers; Surface Plasmon Resonance, Mass spectrometry, Chromatography, X-Ray Crystallography, Flow-Cytometry, Recent advances in biophysical techniques – HiTS-FLIP, Mass Photometry, DNA nanotechnology Laboratory: Separation of nucleic acid and proteins on agarose and polyacrylamide gel electrophoresis respectively; difference between native and SDS-PAGE; protein purification using affinity and size exclusion chromatography; molecular weight calculation from SEC; protein purity and secondary structure assessment using CD spectroscopy; characterizing cells or biomolecules using Raman spectroscopy.