BBL744
| BBL744 | |
|---|---|
| Animal Cell Technology | |
| Credits | 4 |
| Structure | 3-0-2 |
| Pre-requisites | |
| Overlaps | |
BBL744 : Animal Cell Technology
[edit]Characteristic of animal cell, metabolism, regulation and nutritional requirements; Kinetics of cell growth and product formation and effect of shear force; Product and substrate transport; Perfusion bioreactors, Hollow fiber bioreactor, Operational strategies and integrated approach; Micro and macro carrier culture; Hybridoma technology; Genetic engineering in animal cell culture; Scale-up and large scale operation; Case studies. Laboratory: Cell culture in static phase (T-flask), quantification of cell growth, monolayer culture, determination of critical shear stress, micro carrier, Cell viability assay. Case studies to understand growth kinetics and product kinetics in actul cell culture system.