BBL433
| BBL433 | |
|---|---|
| Enzyme Science and Engineering | |
| Credits | 4 |
| Structure | 3-0-2 |
| Pre-requisites | BBL431 |
| Overlaps | |
BBL433 : Enzyme Science and Engineering
[edit]Introduction and scope; Chemical and functional nature of enzymes; Application of enzymes in process industries and health care; microbial production and purification of industrial enzymes; kinetics of enzyme catalysed reactions; immobilization of enzymes; stabilization of enzymes. Bioreactors for soluble and immobilized enzymes, mass transfer and catalysis in immobilized reactors. Enzyme based biosensors; enzyme catalysed process with cofactor regeneration; Enzyme reactions in micro-aqueous medium and non-conventional medium. Laboratory: Assay of enzyme activity and specific activity; kinetic analysis of an enzyme catalysed reaction; Immobilization of enzymes by adsorption and covalent binding; salt precipitation of an enzyme; immobilization of microbial cells by entrapment; effect of water and solvent on the lipase catalysed esterification reaction.