BBL231
| BBL231 | |
|---|---|
| Molecular Biology and Genetics | |
| Credits | 4.5 |
| Structure | 3-0-3 |
| Pre-requisites | BBL131, BBL132 |
| Overlaps | |
BBL231 : Molecular Biology and Genetics
[edit]Historical development and essentials of Mendelian genetics. Chromosomal theory of inheritance. Evolution and development of molecular biology. DNA model and classes. Organization of eukaryotic chromosome – the chromatin structure. Gene structure and Genome. Transposon. Genetic Information and its perpetuation – DNA replication, damage and repair. Telomere and Aging. Transcription, translation. Molecular biology of bacteriophage lamda. Gene exchange in bacteria. Gene regulation in prokaryotes. The operon model – lac, ara, trp operons and gene regulation. Gene Regulation in Eukaryotes. DNA Methylation and Genomic Imprinting. Laboratory: Isolation of genomic and plasmid DNA, Agarose Gel Electrophoresis of DNA, Restriction digestion of DNA, RNA isolation, Primer design, PCR, RT-PCR, Competent cell preparation and Transformation, Gene Induction.