SBD895

SBD895
MS Research project
Credits	36
Structure	0-0-72
Pre-requisites	CML101 or SBL100 or BBL131 for UG students.
Overlaps

SBD895 : MS Research project

The research problem will be assigned by the supervisor. It is expected that the student will undertake the problem early in the program. SBV895 Developmental Biology 1 credit (1-0-0) Overview of the principles of animal development: how a single- celled fertilised egg becomes an embryo and then a fully formed adult body; Understanding role of gene networks: how does the control of gene regulation contribute to development? Cell fate and differentiation: overview of the mechanisms of developmental commitment and maintenance of cell differentiation; Patterning: how are the axes formed? Organogenesis: how do developmental processes affect tissue homeostasis and restoration? Growth and post-embryonic development: aging and senescence; Regeneration: how can mechanisms of development be re-employed? Comparative development and evolution: how does comparison between species explain the emergence of new features? Experimental developmental biology: model organisms and the main techniques to study development.SBV896 Single Molecule Biophysics 1 Credit (1-0-1) Introduction to the course; Entropy and free energy – Boltzmann distribution, mass action law etc; Single molecule vs Bulk; Magnetic and Optical tweezer; Single molecule studies of nucleic acids and their proteins; Cellular mechanochemistry; Raman spectroscopy, Fluorescence; Crossbreeding of tweezing techniques with spectroscopy. SBV897 Angiogenesis in Health and Diseases 1 Credit (1-0-0) Angiogenesis and vasculogenesis; the lymphatic system; introduction to blood vessels: types, structure, and composition. Blood vessel components – endothelial cells, pericytes, and vascular smooth muscle cells; vascular endothelial growth factor (VEGF) biology – its role in angiogenesis and vascular permeability; basic fibroblast growth factor biology; VEGF and FGF receptors, tyrosine kinase signaling and activation; angiogenesis and immune system; tumor angiogenesis - Judah Folkman hypothesis, discovery and how the field of vascular biology in cancer has evolved over the years; tumor vasculature and perfusion; antiangiogenesis as a current therapeutic strategy – where we stand?; blood vessel inhibition vs normalization; vessel co-option and vascular mimicry; atherogenesis; anti-angiogenic therapy in age-related macular degeneration; Vascular aging; different in vitro, in vivo, and ex vivo models to study vascular biology. SBV898 Techniques in Mammalian Cell Culture 1 Credit (1-0-0) for PG students Introduction and historical perspective to animal cell culture, culture conditions, cell line characterization, scale-up, biosafety levels, techniques for genetic manipulation of cells including viral transduction and CRISPR/Cas editing, advanced techniques such as iPSC production and culture, embroids and organoids. SBV899 Assays in Drug Development 1 Credit (1-0-0) None for PG students. Protein and nucleic acid quantification assays- spectrophotometric- and fluorometric methods, qPCR. Assays for monitoring receptor-drug interaction – radioactivity-, fluorescence-, luminescence-, surface plasmon resonance (SPR)- and flow cytometry-based assays. Enzyme kinetics and inhibition assays – Basics of enzyme Kinetics, biochemical assays for miscellaneous therapeutically relevant enzymes, different modes of enzyme inhibition and assays for studying the mechanism of action, high-throughput screening assays. Immunoassays- radioimmunoassay (RAI), enzyme-linked immunosorbent assay (ELISA), miscellaneous applications. Protein-protein interaction inhibition assays – ELISA-type, fluorescence-based and immunoprecipitation assays for monitoring inhibition of protein-protein interaction. Cell viability and cytotoxicity assays – assays relying on reduction of tetrazolium and resazurin compounds, activity of protease markers, ATP detection, real-time assay for viable cells, dye- and marker-based analysis of dead cells. Cellular thermal shift assay (CETSA) – basic principle and miscellaneous applications, high throughput CETSA, MS-CETSA. Assays for ADME/Pharmacokinetic parameters – Assays for evaluating lipophilicity, aqueous solubility, microsome stability, plasma stability, plasma protein binding, membrane permeability, cytotoxicity, cytochrome P450 inhibiton, tmax, Cmax, t1/2, area-under-curve (AUC) etc. 357