SBp810
| SBp810 | |
|---|---|
| Advanced Bioscience Techniques | |
| Credits | 2 |
| Structure | 0-0-4 |
| Pre-requisites | EC 75 and SBL100 or Equivalent |
| Overlaps | |
SBp810 : Advanced Bioscience Techniques
[edit]Particle sizing, biological and biomolecular visualization tools, Courses of Study 2024-2025 Biological Sciences 355advanced and analytical spectrometry, cell and molecular separation techniques, DNA and protein interaction techniques, membrane interaction and signalling, bioreactors, tissue culture, transgene technology, electrophysiology methods. SBV881 Advances in Chemical Biology 1 Credit (1-0-0) Structural aspects of proteins and nucleic acids, Mechanism of action of biological molecules, Chemical approaches to solve biological problems, Designing chemical tools for addressing problems in biology, Bioconjugate chemistry, Recent developments in these areas. SBV882 Biological Membranes 1 Credit (1-0-0) Introduction to the hydrophobic effect, Phospholipid model systems, Cellular membrane asymmetry, Membrane dynamics, Membrane trafficking, Membrane fusion, Membrane proteins (Form and function), Small molecule permeability, Pores channels and transporters, Lipid systems for drug delivery. SBV883 Chaperone and protein Conformational Disorders 1 Credit (1-0-0) Molecular mechanism of protein misfolding, fate of aggregated proteins in the cell, various protein misfolding disorders in humans, mechanism of action of molecular chaperones in various cells, chaperone assisted suppression of protein misfolding. SBV884 Elements of Neuroscience 1 Credit (1-0-0) Introduction to cell biology of neurons; presynaptic and post synaptic mechanisms; signal transduction cascades; neural integration; Hodgkin-Huxley experiments; Na and K pumps; physiological significance of pump modulation; Na and K channels; type and function of different Ca activated K channels; structure function and inactivation; tools for studying Ca signalling; caging and releasing Ca in the neurons; role of nitric oxide; Long term potentiation. SBV885 protein Aggregations and Diseases 1 Credit (1-0-0) Introduction to protein aggregation (amorphous and amyloid), types of aggregates, difference between aggregation and precipitation; External and internal factors for protein aggregation, pH, temperature and protein concentration effects; hydrophobicity, discordant helices; Structural and conformational prerequisites of amyloidogenesis, predominance of beta-sheet, alpha-helices or random coils of native protein; generic nature of protein folding and misfolding, Cytotoxic intermediates in the fibrillation pathway, Oxidative stress and protein deposition disease, Protein aggregation, ion channel formation, and membrane damage, Recent trends in prevention of amyloidosis; drugs, antibodies, combination therapy. SBV886 Signaling pathway Analysis 1 Credit (1-0-0) Introduction to modelling of biological systems – history, types of models, macroscopic phenomena, modelling of cellular systems; hierarchy in information transmission and utilization, interaction between different levels of information leading complex behaviour; robustness of cellular systems and its significance; molecules that transmit signals, role of signaling in regulation of cellular functions, gene regulation; signal transduction – evolution and history; first messengers and receptors, GTP-binding proteins; Calcium Signaling – free, bound and trapped calcium, mechanisms regulating calcium concentration, calcium changes in single cells; protein phosphorylation as a switch, protein kinase A, protein kinase C, structure of signaling pathways, extracting motifs from pathways, relating motifs to observations; dynamics and periodicity in signaling pathways. SBV887 Current Topics in Computational Biology 1 Credit (1-0-0) Bring about awareness of the challenges in Genomics, Proteomics, Metabolomics and Structural Biology. SBV888 Current Trends in Computer Aided Drug Discovery 1 Credit (1-0-0) Teach students various methods for target identification, and applications QSAR and molecular modelling in drug discovery. SBV889 Diagnostic Virology 1 Credit (1-0-0) Introduction to diagnostic virology – direct and indirect methods, specimens and window period; Microscopy – light microscopy, electron microscopy, and fluorescence microscopy in virus identifications; Methods of virus isolation – cell culture, embryonated egg inoculation and animal inoculation; Viral antigen detection – methods, assay characteristics, rapid antigen identification techniques; Detection of viral antibodies – methods, role of quantitative measurements, class-specific immunoglobulin detection; Viral nucleic acids – amplification, detection and quantitation methods; Molecular epidemiology of viral infections – high throughput methods; Identifying antiviral resistance – genotypic and phenotypic approaches; Quality control in diagnostic virology – internal and external quality control, international standards, and Shewhart control charts. SBV890 Kinetoplastid parasites and Novel Targets 1 Credit(1-0-0) Kinetoplastid diseases, transmission, clinical features, immune evasion, treatment, antimicrobial chemotherapy, drug resistance, cross – resistance, Leishmania, promastigotes and amastigotes, procyclic and metacyclic, macrophage, interaction with sand fly, cytokine response, transmission, syndromes associated with leishmaniasis, microtubules in kinetoplastida, dynamics and posttranslational modifications, drug interactions, resistance against tubulin binding agents, arsenite resistance in Leishmania, transporters in kinetoplastid protozoa and drug targets, leishmanial glucose transporters, function of histone deacytylases in kinetoplastid protozoa, DNA – topoisomerases in Leishmania, a possible therapeutic target, exoproteome of leishmania, importane and its application in Leishmania. SBV891 – Virus Host Interactions 1 Credit (1-0-0) Introduction to the virus life cycle; host cell surface molecules utilized as virus receptors, mechanism of cellular membrane penetration for enveloped and non-enveloped animal viruses, cellular entry of bacteriophages and plant viruses; icosahedral and helical capsids, disassembly and transport of genome to the replication site, process of replication, modification of cellular organelles and hijacking of host cell resources; site and manner of progeny virus assembly ; lytic and lysogenic viruses; virus egress and involvement of the host secretory pathway; host defence mechanisms, virus strategies to evade host immune system, antiviral therapies and drug discovery. SBV892 Current Topics in Ion Channels and Receptors 1 credit (1-0-0) Courses of Study 2024-2025 Biological Sciences 356Overview of neuronal physiology; Ion channels and receptors of excitable cell membrane; Ion channel properties: Permeability, Selectivity, Gating and Rectification; Nernst-Plank and Goldman-Hodgkin-Katz equations; Classification of ion channels and receptors; Structure and function studies of ion channels and receptors: voltage-gated Na+ and K+ channels, ligand-gated acetylcholine receptor channel, mechano-gated mscL and Piezo channels; Allosteric modulation of ion channels: theories and application; Ion channel and receptor pharmacology; Methods to study ion channels and receptors: Patch-Clamp, planar-bilayer, crystallography and Cryo-EM studies; Simulation of single channel activity and macroscopic currents from kinetic modeling. SBV893 Cellular Differentiation and Therapy 1 credit (1-0-0) Introduction to differentiation including concepts of totipotency, pluripotency (stem cell), multipotency. Examples of the fertilized egg, somatic stem cells and plant callus will be included. Differentiation vis-a-vis cellular proliferation. Embryonic differentiation and the formation of the three germ layers. Fate specification by growth factors, concept of microenvironment and mechanotransduction in differentiation. Examples of the haematopoietic system. Nuclear signatures and differentiation. Cellular reprogramming–concepts and methodologies used including dedifferentiation - induced pluripotent stem cells (iPSC). Differentiation and its association with diseases such as Leukemia. How differentiation may be used in therapy. Differentiation in the large-scale production of specific cell types. Future perspectives. SBV894 Current Methods in Mammalian Genetics 1 credit (1-0-0) Introduction to mouse genetics – genotypes, genetic backgrounds and methods; Conventional methods of gene targeting and transgenics – gene delivery into embryonic stem cells or zygotes, gene-traps, breeding strategies; Establishment of cell culture models from mice – Fibroblasts and macrophage cultures, rescue strategies using retro/lentiviral transduction; Gene targeting in cells using CRISPR/Cas9 - mutations, activation and suppression; In vivo conditional knockout strategies using Cre/lox and Flippase/FRT system; Bone-marrow chimeras; Animal models of human diseases.