BBL731
| BBL731 | |
|---|---|
| Bioseparation Engineering | |
| Credits | 4.5 |
| Structure | 3-0-3 |
| Pre-requisites | BBL331, BBL432, BBL433 |
| Overlaps | |
BBL731 : Bioseparation Engineering
[edit]Revision of mechanical separation (filtration, Centrifugation etc.), cell disruption, Protein precipitation and its separation, Extraction, Adsorption Desorption processes, Chromatographic methods based Courses of Study 2024-2025 Biochemical Engineering and Biotechnology 123123on size, charge, shape, biological affinity etc., Membrane separations- ultrafiltration and electrodialysis, Electrophoresis, Crystallization, Drying, Case studies. Laboratory: Conventional filtration batch & continuous, Centrifugation in batch and continuous centrifuge, Cell disruption, Protein precipitation and its recovery, Thin Layer Chromatography (TLC), Membrane based filtration-ultrafiltration in cross. Flow modules and microfiltration, electrodialysis, Adsorption Column Studies and Freeze Drying Studies.Laboratory: Cell disruption through various techniques, Filtration to understand Darcy's law, Exposure to continuous filtration process, Understanding the centrifugation principles and calculate settling velocity, Adsorption of dyes to understand the break-through curves, Application of solvent extraction principles, Experience in packing the column for gel extraction chromatography, operation of gel filtration chromatography, Hands-on experience on HPLC, Construction of SMB process.