https://wiki.devclub.in/index.php?action=history&feed=atom&title=SBL540 2026-04-09T10:34:21Z Revision history for this page on the wiki MediaWiki 1.45.1 https://wiki.devclub.in/index.php?title=SBL540&diff=2277&oldid=prev 2026-03-04T10:23:24Z

<p>Creating course page via bot</p> <p><b>New page</b></p><div>{{Infobox Course<br /> | code = SBL540<br /> | name = Experimental Methods in Biology<br /> | credits = 4<br /> | credit_structure = 3-0-2<br /> | pre_requisites = <br /> | overlaps = SBL721<br /> }}<br /> <br /> == SBL540 : Experimental Methods in Biology ==<br /> Courses of Study 2024-2025 Biological Sciences 350Introduction to biomolecules and cells; Electrophoresis, DNA fingerprinting and EMSA; Basics of microscopy; Optical Spectroscopy – Raman, IR, Fluorescence, UV-VIS; FCS; Electron Microscopy; NMR; Circular Dichroism; Force Spectroscopy – AFM, Optical Tweezers, Magnetic Tweezers; Surface Plasmon Resonance, Mass spectrometry, Chromatography, X-Ray Crystallography, Flow-Cytometry, Recent advances in biophysical techniques – HiTS-FLIP, Mass Photometry, DNA nanotechnology Laboratory: Separation of nucleic acid and proteins on agarose and polyacrylamide gel electrophoresis respectively; difference between native and SDS-PAGE; protein purification using affinity and size exclusion chromatography; molecular weight calculation from SEC; protein purity and secondary structure assessment using CD spectroscopy; characterizing cells or biomolecules using Raman spectroscopy.</div>

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